Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Abstract Nicotine, a toxic and addictive alkaloid from tobacco, is an environmental pollutant. However, nicotine-degrading bacteria (NDB) their function in tobacco planting soil are not fully understood. First, 52 NDB strains belonging to seven genera were isolated soil. The most dominant Flavobacterium (36.5%), Pseudomonas (30.8%), Arthrobacter (15.4%), Chitinophaga have been previously reported. Then, two efficient strains, nitrophenolicus ND6 Stenotrophomonas geniculata ND16, screened inoculated the compost fertilizer waste. nicotine concentrations reduced 1.5 mg/g (DW) below safety threshold of 0.5 mg/g. Furthermore, strain followed pyridine pathway degradation, but degrading ND16 could be determined according genomic analysis color change. Finally, abundance genes rhizosphere was investigated via metagenomic analysis. Five key genes, ndhA , nctB kdhL nboR dhponh represent whole process positively correlated with ( p < 0.05). In conclusion, various including unknown species live degrade efficiently. Some used monitoring degradation environment. fermentation waste promising application NDB. Graphical